DV Biologics total RNA passes an extensive testing to ensure a high degree of purity and intactness. RNA is isolated using a modified guanidinium thiocyanate method. Residual genomic DNA is removed with RNase-free DNase I. DV Biologics total RNA can be used in downstream applications such as RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, primer extension, poly A+ RNA selection, RNase/S1 nuclease protection and microarrays. Mucopolysaccharidoses are a group of metabolic disorders caused by the absence or malfunctioning of lysosomal enzymes needed to break down molecules called glycosaminoglycans - long chains of sugar carbohydrates in each of our cells that help build bone, cartilage, tendons, corneas, skin and connective tissue.