DV Biologics total RNA passes an extensive testing to ensure a high degree of purity and intactness. RNA is isolated using a modified guanidinium thiocyanate method. Residual genomic DNA is removed with RNase-free DNase I. DV Biologics total RNA can be used in downstream applications such as RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, primer extension, poly A+ RNA selection, RNase/S1 nuclease protection and microarrays. Robertsonian translocation (RTL) is a common form of chromosomal rearrangement that in humans that occurs in the five acrocentric chromosome pairs. People with RTL have only 45 chromosomes in their cells with all essential genetic material present. They appear normal, however their children may be either normal and carry the fusion chromosome, or they may inherit a missing or extra long arm of an acrocentric chromosome causing disorders such as Down syndrome.