DV Biologics cDNA is synthesized from a highly pure and intact total RNA that was isolated by modified guanidine thiocyanate technique and devoid of genomic DNA contamination. Five µg of total RNA was reverse transcribed into a single strand cDNA using oligo–dT primer in 20 ul total volume. RT reaction was stopped by heating at 85oC for 5 minutes. Using 1 µl of cDNA per PCR reaction is sufficient for 20 typical PCRs. DV Biologics cDNA can be used in gene expression and cloning studies, analysis of mRNA alternative splicing, gene mutation analysis and other molecular biology fields. Diabetes mellitus type 2 (DT2) is a disorder characterized by high blood glucose that occurs because cells do not respond appropriately when insulin is present (insulin resistance). Studies have shown that there is a strong inheritable genetic connection. If your family members have DT2 this increases your risk for developing it. Severe complications can result from improperly managed DT2 including renal failure, erectile dysfunction, blindness, and coronary artery disease.