DV Biologics total RNA passes an extensive testing to ensure a high degree of purity and intactness. RNA is isolated using a modified guanidinium thiocyanate method. Residual genomic DNA is removed with RNase-free DNase I. DV Biologics total RNA can be used in downstream applications such as RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, primer extension, poly A+ RNA selection, RNase/S1 nuclease protection and microarrays. Diabetes mellitus type 2 (DT2) is a disorder characterized by high blood glucose that occurs because cells do not respond appropriately when insulin is present (insulin resistance). Studies have shown that there is a strong inheritable genetic connection. If your family members have DT2 this increases your risk for developing it. Severe complications can result from improperly managed DT2 including renal failure, erectile dysfunction, blindness, and coronary artery disease.