DV Biologics total RNA passes an extensive testing to ensure a high degree of purity and intactness. RNA is isolated using a modified guanidinium thiocyanate method. Residual genomic DNA is removed with RNase-free DNase I. DV Biologics total RNA can be used in downstream applications such as RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, primer extension, poly A+ RNA selection, RNase/S1 nuclease protection and microarrays. Transverse myelitis is a neurological disorder caused by an inflammatory process of grey and while matter of the spinal cord, which can result in axonal demyelination. The cause of transverse myelitis is not understood, and is considered to arise idiopathically associated with an immune-mediated inflamation following infections or vaccinations, or due to multiple sclerosis.